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KMID : 0895820090190040095
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Journal of Oriental Rehabilitation Medicine
2009 Volume.19 No. 4 p.95 ~ p.113
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Study on Effect of Saengbal-eum -II on Hair Regrowth Promotion in C57BL/6 Mice
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Han Ae-Ri
Sohn Nak-Won
Chung Seok-Hee
Kim Sung-Su
Song Mi-Yeon
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Abstract
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Objectives: Saengbal-eum-II(Sh?ngfa-y?n-II)is a hair care product which is composed of ten plant extracts used in oriental medicine. This study was carried out to investigate the effects of Saengbal-eum-II(Sh?ngfa-y?n-II)on hair regrowth and cytokine changes in a shaving model of C57BL/6 mice.
Materials and Methods: Five-week-old mice were acclimated for 1 week at a temperature between 21-23℃, 40-60% relative humidity, and 12 h of alight/dark cycle before beginning of the experiment. There were three experimental groups including 50% ethanol(EtOH, control), a positive control of 3% Minoxidil, and 30% Saengbal-eum-II(Sh?ngfa-y?n-II) in 50% ethanol in 18 female mice. The test compounds were topically treated once a day over 12 days. The hair regrowth was photographically and histologically determined during the experimental period of 12 days. Revelation of EGF, TGF-β 1 and IL-6 in hair follicle were also determined using immunohistochemistry. In addition to that, IL-6, TNF-α, and IL-1β in skin tissue were determined using enzyme-linked immunosorbent assay (ELISA).
Results: Hair regrowth in 3% Minoxidil and Saengbal-eum-II(Sh?ngfa-y?n-II)groups was promoted earlier and faster than the control group. Concentrations of hairs and thick-hair ratio in 3% Minoxidil and Saengbal-eum-II(Sh?ngfa-y?n-II) groups were promoted than the control group. EGF was moderately positive in hair follicle of 3% Minoxidil and Saengbal-eum-II(Sh?ngfa-y?n-II) groups, but negative in the control group. TGF-β1 was not significantly difference between the groups. IL-6 in hair follicle of Saengbal-eum-II(Sh?ngfa-y?n-II) group was negative, but weakly positive in 3% Minoxidil and control group. IL-6 and IL-1β in skin tissue were significantly decreased in Saengbal-eum-II(Sh?ngfa-y?n-II)group, but there was not significantly decreased in 3% Minoxidil and control group. TNF-α in skin tissue was significantly decreased in 3% Minoxidil and Saengbal-eum-II(Sh?ngfa-y?n-II)groups.
Conclusions: These results suggest that Saengbal-eum-II(Sh?ngfa-y?n-II)has hair growth promoting activity and it can be used for treatment of alopecia. And these effects relate to EGF revelation of hair follicle and a decrease IL-6, TNF-α, and IL-1β in skin tissue.
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KEYWORD
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Alopecia, C57BL/6mice, Saengbal-eum-II(Sh?ngfa-y?n-II), Cytokine, Hairregrowth
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